Genetics and molecular biology, 22, 4, 591599 1999theory and analysis of partial diallel crosses 591 introduction a diallel is a mating system that involves all possible crosses among a group of parents. Diallel analyses can give different results depending on the model and the experimental design. Using a diallel mating design, this study focuses on. Thereafter, for the first cross combination, h1 is referred to as the first cross in the half diallel, i. Diallel analysis for morphological and biochemical traits. We performed genetic analyses of growth, carbon isotope discrimination ac, and foliar n concentration.
Pdf estimating effects of a single gene and polygenes on. The experimental design consisted of a randomized block, with 5 replications and 3. Standard commercial statistical packages do not allow direct specification of the diallel mating model and therefore are not capable of analysing the diallel mating design from existing models or procedures, for balanced and unbalanced diallel. General and specific combining ability from partial. Analysis of diallel mating designs nc state university. Robustness of complete diallel cross plan using partially. Thus, when an autotetraploid species is analyzed in a diallel mating design, a comparison of the estimates of sca and gca helps determine the importance of trigenic and tetragenic loci. Before sowing, the filled seeds were separated from empty seeds immersing each seedlot. Expectations of the sums of squares of a quadratic analysis of the observations lead to estimates of design components of variance and covariance which can be interpreted genetically and environmentally. In diallel crosses, the specific combining ability sca component contains additive effects, a portion of the digenic variance, and the trigenic and tetragenic effects and their resulting interactions. Maize streak virus disease, grain yield, resistance, full diallel, general combining ability, specific combining ability. Simplest mating design providing both full sibs and half sibs. The procedure gives rise to a maximum of p2 combinations.
Parents, one set of 1 and reciprocal 1 are included. In a partial diallel mating design only some of the possible pairs of crosses are made, and there are many variants of partial diallels that make more or fewer crosses. The half diallel mating design, particularly a series of disconnected half diallel mating design has been widely adopted as a mating design in many commercially important tree species for estimating the variances of general gca and specific sca combining abilities, heritability, and genetic correlation. Diallel analysis and its applications in plant breeding pdf. Therefore, the objectives of this study were to determine the gca, sca and heritability of tomato genotypes crossed in a half diallel mating design. Introduction griffings analysis hayman and jinks analysis variety cross diallel partial diallel cross discussion statistical programssoftware summa. The ppartial twolevel diallel mating design the ipartial twolevel diallel mating design 52 56 4. By a complete diallel cross cdc mating design is one in which a set of p inbred lines is chosen and crosses among these lines are made. Pdf mating designs commonly used in plant breeding. Miranda 1988 the diallel mating design has been used and abused more extensively than any other in maize and other plant species. This general definition is necessary because a diallel.
Griffing 1956 gave four classifications of cdc mating designs as. If reciprocal effects were considerable in such a design, it would. From the diallel mating design, plant breeders can estimate general combining ability gca and specific combining ability sca. A diallel cross is a mating scheme used by plant and animal breeders, as well as geneticists, to investigate the genetic underpinnings of qualitative and quantitative traits. This genetic design is used to study polygenic systems that determine quantitative traits. Parental selection in diallel crosses of jatropha curcas. Oct 03, 2017 the diallel mating design has been successfully applied in the plant breeding studies over more than 50 years. Diallel analysis of yield components of snap beans exposed to two temperature stress environments. The objectives of this thesis are to develop and evaluate the statistical methods to detect the major genes controlling quantitative traits in tree breeding population using simulated data and phenotypic data derived from a half diallel mating design. Additive main effect and multiplicative interaction model for a diallel. Some issues which evidently remain as areas of contention in diallel analysis are reveiwed. Provides sca of each cross but provides gca of both lines and testers. Sprague and tatum 1942 introduced the diallel cross concept to plant. Analysis of the diallel mating design for maize inbred lines acsess.
However, there are several factors affecting the choices of mating designs. Study on combining ability and heterosis in maize zea. Analysis of diallel mating designs fikret isik north carolina state university, raleigh, usa 6. One can only agree with hallauer and 1988 when they state that the diallel mating design has been used and abused more extensively than any other. Progeny test and experimental design one hundred seedlots from the controlled crossings were collected in october 1990. Two kinds of statistical linear models are commonly employed to analyze diallel crosses with balanced or unbalanced data. Pdf combining ability studies using diallel mating.
Abstract mating designs are the study of progenies developed through various methods like diallel cross plans which are subjected to incomplete block designs. Proper analysis of the diallel mating design iowa state university. Diallel analysis for morphological and biochemical traits in. The procedure requires two runs of proc and varcomp and results in estimates of additive and nonadditive genetic variation.
The diallel mating design has been used and abused more extensively than. With the exception of diallel 1, the allocation of 875 parents to diallel crosses. Pdf combining ability studies using diallel mating design. Unlike most other mating designs, which can only provide estimates of genetic variance components, the diallel can provide information about the combining. I a practical analysis procedure for anova approximati.
The concept of robustness in a design has been studied and available in the literature. Mar 29, 2016 in contrast, the diallel and selective mating aspects of the new system force the simultaneous insertion of multiple genotypes into a few central populations. Subsequently, mating designs were developed to estimate different genetic components of. Genetic variation in growth, carbon isotope discrimination. Analyses of halfdiallel mating designs with missing crosses. Hello friends, i am vikas mangal, ars scientist genetics and plant breeding crijaf, barrackpore. Diallel crossing technique in cotton has been used by other cotton breeders also to get the desired results i. Pdf application of diallel analyses in crop improvement. All 21 sets involved reciprocal crosses in order to make up the desired half diallel design. Quantitative trait mapping in a diallel cross of recombinant inbred lines shirngwern tsaih,1 lu lu,2 david c. There are four main types of diallel mating design. Complete diallel mating designs are generally not necessary, and the partial half diallel scheme is usually employed by most tree breeding programs. The quadratic analysis can be viewed as resulting from a sequential. Pdf utilization of diallel analyses for heritability, gca.
The diallel mating design is defined as making all possible crosses among a group of genotypes. Phenotypic data of a diallel mating under the direction of drs. Inbred parents and their f1 single crosses with a check were evaluated to assess the role of general and specific combining ability and heterosis for some quantitative traits. Two types of analysis are commonly used in a diallel. We, therefore, present a general diallel model and the required additional restrictions for the genetic variancecovariance structure to become equivalent. Full diallel in which parents and reciprocal crosses are involved along with f1 ii. Procedures to analyze half diallel mating designs using the sas statistical package are presented. A method of population improvement of autogamous species that involves parental diallele series, f1 diallale series and selective mating series in the breeding procedure. May 25, 2016 hallauer 100 suggested the nc ii as a preferable design which can make use of a larger number of parents resulting in a fewer crosses generation than a diallel mating design. Diallel designs, analyses, and reference populations heredity. Precision of estimates of genetic variance for any mating design depends on.
Here are some commonly used diallel mating designs in. Griffing 1956 made the best use of diallel mating design for the choice of better parents to be used in hybridization programme. A diallel table is an arrangement in a square of n2 measurements corresponding onetoone to the mating combinations of a diallel cross, each row and column of the square corresponding to offspring with a common parental genotype. Mating design refers to the procedure of producing the progenies, in plant breeding. Interpreting mating designs is an approach to elucidating gene action for yield, even though the designs were developed under the assumption that inheritance is disomic. Before sowing, the filled seeds were separated from empty seeds immersing each seedlot in 90%. The construction of an incomplete environmental design for a. Diallel mating of the selected 875 parents the final selected group of 95 875s was mated in a modified, disconnected, 5parent, partial half diallel design totalling 18 diallels and 154 fullsib families. From the practical point of view, diallel mating designs provide a very simple and convenient method for the estimation of genetic parameters 44, 45.
However, this mating design can fit into, at least. Variances for general combining ability gca and specific combining ability sca and the relationship between midparental gca and sca effects were estimated for tree diameter dbh from a series of 20 sets of 6. Venture were selected for differential temperature tolerance and used as parents in a complete diallel mating design. Diallel crossing is one such important mating system enjoying universal appli cation in plant breeding. Diallel mating design based on the general linear model framed by grif. Here are some commonly used diallel mating designs in forestry. Comparison of the efficiency among half diallel methods in. Jul 26, 2017 diallel mating design is used to evaluate several inbred lines in terms of combining ability variances and effects. Principles and utilization of combining ability in plant. General and specific combining ability from partial diallels. Since, 1950 this study was further aggravated to estimate genetic. A full diallel mating design according to griffings 1956 method 1 was carried out.
The effects of missing blocks on complete diallel cross designs are examined in this study. Combining ability studies using diallel mating design in indian mustard brassica juncea l. Diallel mating design first presented by schmidt 1919 became an important tool used to produce. Dec 18, 2016 line x tester design an extension of top cross design more than one testers are used in lt mating design involves hybridization between lines f and wide based testers in one to one fashion. One of the assumptions required to estimate genetic parameters by use of the diallel mating design is that the genes in the parents are independently distributed.
The eleven genotypes were crossed in a diallel mating design during februarymarch 2012, resulting in. Diallel analysis and genetic diversity analysis assist in the. To characterize a optimal rowcolumn designs for complete. Gca values of the parents were estimated with best linear prediction blp solutions using the family means. This design has been applied in plant breeding for selection of testcross performance. Genetic variation in growth, carbon isotope discrimination, and foliar n concentration in picea mariana. A set of crosses produced by involving n lines in all possible combinations is designated as diallel cross and the analysis is known as diallel analysis. Diallel mating design is used to evaluate several inbred lines in terms of combining ability variances and effects.
Significant sca variance for dbh was almost equal to gca variance for the combined analysis. Diallel cross refers to mating of selected parents in all possible combinations and evaluation of a set of diallel crosses is known as diallel analysis. Diallel analysis and its applications in plant breeding. Diallel is a greek word and introduced by schmidt in 1919. Application of diallel analyses in crop improvement. When the same parents are used as females and males in breeding, the mating design is called diallel. Mating design refers to the procedure of producing the progenies, in plant breeding, plant breeders and geneticists. Pdf utilization of diallel analyses for heritability. Analyses of halfdiallel mating designs with missing. Diallel designs, analyses, and reference populations. More crosses per parent mean more precision for estimating genetic parameters objective 2, for ranking the parents objective 1, and for achieving more gain from making.
Cdc method 1 mating design contains p2 crosses, cdc method. Reciprocal diallel crosses impact combining ability, variance. Full diallel in which parents and reciprocal crosses are involved along with f 1 ii. Diallel analysis and genetic diversity analysis assist in.
Analysis of halfdiallel mating design with missing. G riffing 1956 described statistical models for analyzing four different diallel mating designs method 1, method 2, method 3, and method 4. The diallel mating design has been used successfully for over 50 years in plant breeding to estimate the relative combining ability of lines. The interpretation of results from these investigations is based upon a statistical analysis of the data. Because of the availability of clearly defined general combining ability gca and specific combining ability sca effects and reciprocal effect rec and easy to understand statistical models as well as the availability of software to analyze diallel. Diallel analysis for yield and its components in okra. The eleven genotypes were crossed in a diallel mating design during februarymarch 2012, resulting in fiftyfive one way f 1 hybrids.
Diallel mating design has been used to create variability and to know the genetic parameters controlling the inheritance of the characters under study. Combining ability of tropical early maize zea mays l. New software and algorithms to analyse diallel mating. The attached copy is furnished to the author for noncommercial research and education use, including for instruction at the authors institution, sharing with colleagues and providing to institution. A unified framework to fit and compare different models by mixed.
This is the case in 21 sets of a 6 x 6 half diallel mating scheme for radiata pine australiawide in the early 1980s. Introduction research in quantitative genetics is based to a large extent upon experimental investigations. In the estimation of genetic variance components for an ancestral reference population in equilibrium. Diallel analysis of agronomic, physiological and metabolite. Types of diallel analysis depending upon whether or not the parental inbreeds or the reciprocal 1 are included or both, there are four type of diallel crossing system griffing, 1956. Reciprocal diallel crosses impact combining ability. Mating designs and their implications for plant breeding. Journal of plant breeding and crop science genetic. Diallel analysis of yield components of snap beans exposed to. Churchill1 1the jackson laboratory, 600 maine st, bar harbor, maine, 04609, usa 2center of genomics and bioinformatics and the department of anatomy and neurobiology, university of tennessee, health science center, 855 monroe ave. Diallel analysis of yield components of snap beans exposed.
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